PerkinElmer
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EGF (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® EGF Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor (EGF) in cell culture media, serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
AL339HV 100 assay points 626.00 USD
AL339C 500 assay points 1476.00 USD
AL339F 5,000 assay points 9800.00 USD
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Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Epidermal Growth Factor (EGF) is a 6.2 kDa protein ligand that binds with high affinity to the epidermal growth factor receptor. Initially EGF is expressed as pro-EGF and is later cleaved to its mature form by enzymes and released via exocytosis. EGF, through EGFR, has been observed to play a role in initiating cell growth, proliferation and differentiation. Dysregulation of EGF levels has been linked to several diseases including but not limited to ovarian cancer and diabetes.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target EGF
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet Manual: AlphaLISA EGF Detection Kit AL339 PDF  777 KB
Event Society of Laboratory Automation & Screening (SLAS)