PerkinElmer
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EGF/EGFR AlphaLISA Binding Kit, 500 Assay Points

The AlphaLISA® EGF/EGFR Binding Kit is designed for the detection of binding activity between Human EGF and EGFR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit the EGF/EGFR interaction.

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Part Number Unit Size List Price Your Price Quantity
AL366HV 100 assay points 675.00 USD
AL366C 500 assay points 1433.00 USD
AL366F 5,000 assay points 9500.00 USD
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Overview

This kit is designed for the detection of binding activity between EGF and EGFR using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of therapetics by using competitive binding.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In this AlphaLISA assay, a biotinylated EGF binds to the Streptavidin-coated Alpha Donor beads, while EGFR-Fc is captured by Anti-Human IgG Fc-specific AlphaLISA Acceptor beads. When EGF is bound to EGFR, Donor beads and Acceptor beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfers in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Epidermal Growth Factor Receptor (EGFR, Her1, ErbB1) is a 134 kDa cell surface receptor, part of a four member subfamily of receptor tyrosine kinases (erbB1, erbB2, erbB3, and erbB4). EGFR binds to a family of proteins called the epidermal growth factors (primarily EGF), which, upon association induces dimerization of the receptor and initiates signal transduction for the promotion of cell survival and growth. Mutations that cause aberrant overexpression of EGF and/or EGFR have been linked with many different cancers such as non-small-cell lung and colon cancers. Drugs are being developed as a means to better treat these types cancer.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target EGF, EGFR
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual AlphaLISA EGF-EGFR Binding Kit AL366 PDF  505 KB
Event Society of Laboratory Automation & Screening (SLAS)