The AlphaLISA® Host Cell Residual DNA Detection Kit is designed for detection and quantitation of DNA contaminants in cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay. This kit can be used with DNA from different prokaryotic and eukaryotic species.
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Biologics can be impacted negatively by contamination with DNA introduced during fermentation and purification processes. While the use of serum-free media in the manufacturing process significantly improved the success rate on preventing DNA impurities, other routes of contamination, such as microbial contamination, still remain a concern. It is thus critical to remove and monitor DNA impurities at each step in the purification process. This kit is designed to quantify the levels of DNA (from different hosts, either double- or single-stranded and of varying fragment sizes) in cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target||Residual DNA|
|Assay Target Class||DNA|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Host Cell Residual DNA Detection Kit AL331||PDF 577 KB|