The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
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D-dimer is a 200 kDa fragment of fibrinogen and is produced during clot lysis. Fibrinogen is synthesized by the liver and is one of the most abundant proteins in plasma. It has two identical subunits of three polypeptide chains (alpha, beta and gamma). In the coagulation process, fibrinogen is cleaved by thrombin to form the fibrin clot. Then the clot is dissolved through plasmin cleavage of fibrin and forms D-dimer. D-dimer is an important cardiovascular biomarker. It is widely used in diagnosis to exclude deep vein thrombosis (DVT) and pulmonary embolism. Its concentration is also increased after surgery, during pregnancy, and in cancer.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Human D-dimer AL290||PDF 204 KB|
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