PerkinElmer
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CXCL12/SDF-1 (human) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® immunoassay kit for human CXCL12 (SDF-1) enables the quantitative determination of human CXCL12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL3028HV 100 Assay Points 420.00 USD
AL3028C 500 Assay Points 1391.00 USD
AL3028F 5,000 Assay Points 9200.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

CXCL12 is a small protein of 89 amino acids also known as Stromal-Derived Factor (SDF-1). It is a member of the CXC family of chemokines. CXCL12 is a strong chemoattractor for lymphocytes and is involved in angiogenesis in both fetal and adult organisms by recruiting lymphocytes to newly formed vessels. It also plays a role in bone morphology by inhibiting bone formation by osteoclast. Finally, as a natural ligand for the receptor CXCL4, it inhibits binding of HIV1 and 2 to the receptor, thus being a natural anti-AIDS compound.

Specifications

Assay Target CXCL12, SDF1
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 5,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual Manual: AlphaLISA human CXCL12 Detection Kit AL3028 PDF  539 KB