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CXCL1/GRO-α (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human C-X-C Motif Chemokine 1 / Growth-regulated Oncogene alpha (CXCL1 / GRO-α) Detection Kit is designed for detection and quantitation of human CXCL1/GRO-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note this kit has significant cross-reactivity with human CXCL2 and CXCL3.

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AL279C 2745.00 USD

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Overview

One antibody is specific for the cleaved (active) form of caspase-3: rabbit monoclonal antibody, clone number 9H19L2. The second antibody is specific to the full length protein: mouse monoclonal antibody, clone number 4-1-18.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

C-X-C Motif Chemokine 1 (CXCL1) is an 11 kDa chemokine and is part of the intercrine alpha (chemokine CXC) family. The secreted protein is proteolytically processed at the N-terminus and the processed form is usually referred to as GRO-a. CXCL1 is expressed by macrophages, neutrophils and epithelial cells. Its synthesis is induced by a variety of inflammatory mediators such as PDGF, M-CSF, TNFa, LPS, and TLR 4. The major role of CXCL1 is its chemoacttractant activity on neutrophils. CXCL1 plays a role in inflammation and exerts its effects on endothelial cells in an autocrine way. CXCL1 is implicated in spinal cord development, angiogenesis, inflammation, wound healing, and tumorigenesis. CXCL1 is overexpressed in invasive bladder cancer and is secreted by human melanoma cells.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target CXCL1
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Data Sheet Manual AlphaLISA Human CXCL1/GRO-a AL279 PDF 247 KB
Poster AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices PDF 273 KB