PerkinElmer
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Cardiac Troponin T (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human cardiac troponin T enables the quantitative determination of cardiac troponin T in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL3011HV 100 Assay Points 600.00 USD
AL3011C 500 Assay Points 2596.00 USD
AL3011F 5,000 Assay Points 18000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cardiac troponin T is one of three subunits that form the troponin complex of the thin filaments of striated muscle. It is a cardiac regulatory protein that controls the interaction between actin and myosin filaments to modulate the contraction of striated muscle. Cardiac troponin binds to tropomyosin and helps to position the troponin complex on actin filaments. Cardiac troponin T is expressed only in the heart, and patient serum levels are used as a diagnostic marker for myocardial infarction.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Cardiac Troponin T
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cardiovascular
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual Cardiac Troponin T (Human) AlphaLISA Detection Kit PDF  568 KB
Event Society of Laboratory Automation & Screening (SLAS)