The AlphaLISA® Cry2A Detection Kit is designed for detection and quantitation of Cry2A in GMO seeds, leaves, and plant extracts in a homogeneous (no-wash steps, no separation steps) assay.
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Cry2A is an insecticidal protein and is produced by the naturally occurring soil bacterium Bacillus thuringiensis subsp. kurstaki (Btk). The protein is used extensively in agricultural farming as a direct application pesticide. The gene encoding Cry2A has been widely introduced to genomes of many crops (e.g. corn, cotton, and soybean) to create genetically modified organisms (GMO). During growth, genetically modified crops produce the Cry2A protein that confers protection against certain insect pests.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Cry2A Assay Kit AL403||PDF 359 KB|