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Cry1F AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.

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Product Number Unit Size List Price Your Price Quantity
AL402HV 100 Assay Points 565.00 USD
AL402C 500 Assay Points 1384.00 USD
AL402F 5,000 Assay Points 8800.00 USD

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  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cry1F is an insecticidal produced by the naturally occurring soil bacterium Bacillus thuringiensis subsp. kurstaki (Btk). The protein is used extensively in agricultural farming as a direct application pesticide. The gene encoding Cry1F has been widely introduced to genomes of many crops (e.g. corn, cotton, and soybean) to create genetically modified organisms (GMO). During growth, genetically modified crops produce the Cry1F protein that confers protection against certain insect pests.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target Cry1F
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 1 kit
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Agriculture/Plant
Unit Size 5,000 Assay Points

Resource Library & Events

Resource Type File Name File Format
Data Sheet Manual AlphaLISA Cry1F AL402 PDF 419 KB