The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL402HV||100 assay points||582.00 USD|
|AL402C||500 assay points||1426.00 USD|
|AL402F||5,000 assay points||9000.00 USD|
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Cry1F is an insecticidal produced by the naturally occurring soil bacterium Bacillus thuringiensis subsp. kurstaki (Btk). The protein is used extensively in agricultural farming as a direct application pesticide. The gene encoding Cry1F has been widely introduced to genomes of many crops (e.g. corn, cotton, and soybean) to create genetically modified organisms (GMO). During growth, genetically modified crops produce the Cry1F protein that confers protection against certain insect pests.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Data Sheet||Manual AlphaLISA Cry1F AL402||PDF 419 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|