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CRP (rhesus) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® immunoassay kit for rhesus C-reactive protein (CRP) enables the quantitative determination of rhesus CRP in buffered solution, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL563HV 100 Assay Points 420.00 USD
AL563C 500 Assay Points 1391.00 USD
AL563F 5,000 Assay Points 9200.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

C-reactive Protein (CRP) is a 224 Amino-Acid serum protein present in a homopentameric form. The function of CRP is to bind phosphocholines expressed by dead and dying cells to activate the complement system. In response to infection, inflammation, or tissue damage, IL6, IL1, and glucocorticoids induce the production of CRP in the liver. Elevated CRP levels have been identified with acute and chronic inflammatory conditions in general, various types of cancer, rheumatoid arthritis, and cardiovascular diseases like coronary heart disease where the American Heart Association has defined levels of risk based upon CRP levels. Due to CRPs altered expression levels in these diseases, it is a highly valuable biomarker to monitor immune system response. This kit has been designed for the detection of CRP in cynomolgus and rhesus monkey serum and cell culture supernatants.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target CRP
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Rhesus
Therapeutic Area Inflammation
Unit Size 5,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual Manual: AlphaLISA rhesus CRP AL563 PDF  531 KB