AlphaLISA® cGMP kit for quantitative detection of cGMP.
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|Part Number||Unit Size|
|AL316HV||100 assay points|
|AL316C||500 assay points|
|AL316F||5,000 assay points|
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. Cyclic guanosine 3’,5’-monophosphate (cGMP) is a a second messenger molecule with a critical role in signal transduction pathways of most species. cGMP-dependent kinases help in modulation of cGMP dependent ion channels, transporters, and in the alteration of cGMP phosphodiesterases. cGMP levels have also been shown to play a key role in phototransduction in the eye, affecting vascular smooth muscle motility, Nitric Oxide Synthase stimulation, and maintaining electrolyte homeostasis.
This kit contains enough reagents to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume.
In this AlphaLISA competition assay, biotinylated cGMP probe binds to Streptavidin-coated Alpha Donor beads, with anti-cGMP antibody conjugated to AlphaLISA Acceptor beads. In the absence of endogenous cGMP from the sample, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules that diffuse to the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. With increasing concentrations of endogenous cGMP from the sample, fewer Donor and Acceptor beads come together, resulting in a decrease in signal.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Small Molecule|
|Product Brand Name||AlphaLISA|
|Quantity in a Package Amount||500.0 Units|
|Second Messenger Release||cGMP|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Tech sheet AlphaLISA cGMP AL316||PDF 569 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|