The AlphaLISA® Human C-C Motif Chemokine 4 / Macrophage Inflammatory Protein-1beta (CCL4 / MIP-1ß) Detection Kit is designed for detection and quantitation of human CCL4/MIP-1ß in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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C-C Motif Chemokine 4 (CCL4), also known as Macrophage Inflammatory Protein-1beta (MIP-1ß), is a 69 amino acid chemokine signaling through the CCR5 receptor. It is a chemoattractant for natural killer cells, monocytes, and a variety of other immune cells. CCL4 is also an important regulator of macrophage migration. CCL4 is rapidly synthesized by activated T cells, B cells, and monocytes. It is also produced by neutrophils after adhesion to the laminin contained in the basement membrane of blood vessels. It is one of the major HIV-suppressive factors produced by CD8+ T-cells and has been reported to show a direct antiviral activity against HSV-1. Recombinant CCL4 also induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|One Unit Contains||5000 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA human CCL4/MIP-1ß AL258||PDF 209 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|