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CCL3/MCP-1α (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human C-C Motif Chemokine 3 / Macrophage Inflammatory Protein-1alpha (CCL3 / MIP-1α) Detection Kit is designed for detection and quantitation of human CCL3/MIP-1α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number Unit Size List Price Your Price Quantity
AL257C 500 assay points 1433.00 USD
AL257F 5,000 assay points 9500.00 USD

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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

C-C Motif Chemokine 3 (CCL3), also known as Macrophage Inflammatory Protein-1 alpha (MIP-1a), contains 70 amino acids in its mature form. It is a factor produced by macrophages that causes local inflammatory responses in vivo, and induces superoxide production by neutrophils in vitro. CCL3 is involved in the acute inflammatory state and in the recruitment and activation of polymorphonuclear leukocytes. It binds to CCR1, CCR4, and CCR5. CCL3 is known mainly for its activity as a chemotactic cytokine, but may also have a potential role in host defense as a direct antiviral agent. CCL3 has been reported to show a significant direct antiviral activity against HSV-1. It is one of the major HIV-suppressive factors produced by CD8+ T-cells. CCL3 is also an inhibitor of haematopoietic stem cell proliferation.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target CCL3
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Data Sheet Manual AlphaLISA CCL3 / MIP-1a AL257 PDF 252 KB
Poster AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices PDF 273 KB