The AlphaLISA® immunoassay kit for human CCL18 enables the quantitative determination of human chemokine (C-C motif) ligand 18 in buffer, human serum, cell media, and PBMC culture supernatant samples using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
CCL18 is Chemokine (C-C motif) ligand 18, also known as Pulmonary and activation-regulated chemokine (PARC), dendritic cell (DC)-chemokine 1 (DC-CK1), alternative macrophage activation-associated CC chemokine-1 (AMAC-1), and macrophage inflammatory protein-4 (MIP-4). It is a small cytokine belonging to the CC chemokine family and is produced and secreted mainly by innate immune system. But it is not produced by T-cells or B-cells. CCL18 may play a role in both activation of the immune system and the induction of tolerance and homeostasis at steady-state conditions.
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|