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Caspase-3 active AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Caspase-3 Detection Kit is designed for detection and quantitation of active caspase-3 in buffered solution or cell lysates, in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number List Price Your Price Quantity
AL278F 21500.00 USD

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Overview

One antibody is specific for the cleaved (active) form of caspase-3: rabbit monoclonal antibody, clone number 9H19L2. The second antibody is specific to the full length protein: mouse monoclonal antibody, clone number 4-1-18.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Human caspase-3, also known as apopain, is a cysteine protease of 29 kDa. It is widely expressed and is an integral component of the apoptosis cascade. Caspase-3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases. Its cleavage generates a p17 and a p12 subunit. The p17/p12 heterodimer associates with itself to form a heterotetramer. Once active, caspase-3 cleaves key cellular proteins such as poly (ADP-ribose) polymerase, sterol regulatory element binding proteins, and other caspase members. Activation of caspase-3 is commonly used as a biomarker for assessment of apoptosis.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Caspase-3
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Cancer
Unit Size 5,000 assay points