check quantity

AlphaLISA cAMP Detection Kit, 100 Assay Points

AlphaLISA® cAMP kit for quantitative detection of cAMP. This assay can be used as a functional assay for Gs-coupled and Gi-coupled GPCR activity.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Product Number List Price Your Price Quantity
AL315HV 204.00 USD

You successfully added item(s) to your cart

Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

In this AlphaLISA competition assay, biotinylated cAMP probe binds to Streptavidin-coated Alpha Donor beads, with anti-cAMP antibody conjugated to AlphaLISA Acceptor beads. In the absence of endogenous cAMP from the sample, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules that diffuse to the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. With increasing concentrations of endogenous cAMP from the sample, fewer Donor and Acceptor beads come together, resulting in a decrease in signal.

This assay kit contains enough reagents to run 500 wells in 96-well or 384-well format, using a 50 µL final reaction volume (20 µL of sample or cells).

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target cAMP
Assay Target Class Small Molecule
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Second Messenger Release cAMP
Shipping Condition Blue Ice
Therapeutic Area Metabolic
Unit Size 1 kit

Resources, Events & More

Resource Type File Name File Format
Data Sheet Tech sheet AlphaLISA cAMP AL315 PDF 402 KB