The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
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IL-1a is a cytokine produced mainly by immune cells. In particular, IL-1a plays a clinically-relevant role as a mediator of inflammatory responses associated with various diseases such as mastitis, endotoxic shock and bovine tuberculosis. The present kit permits detection of bovine IL-1a (i.e. analyte) in bovine serum, plasma, and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|One Unit Contains||1 kit|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||1 kit|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Bovine IL-1a AL537||PDF 320 KB|