The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1β in bovine serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL539HV||100 assay points||626.00 USD|
|AL539C||500 assay points||2000.00 USD|
|AL539F||5,000 assay points||10000.00 USD|
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IL-1, a proinflammatory cytokine, is produced by immune cells following bacterial, viral, fungal, and parasitic infections. It is produced as two forms, IL-1? and IL-1?. IL-1? mediates both local and systemic inflammatory responses and up- regulation of bovine IL-1? in serum and milk is associated bovine with mastitis diseases and to vaccination. The present kit permits detection of bovine IL-1? (i.e. analyte) in bovine serum, plasma, and cell culture supernatants. Detecting in milk samples is also possible.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 132 KB|
|Data Sheet||Manual AlphaLISA Bovine IL-1ß AL539||PDF 355 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|