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BDNF (human) AlphaLISA Detection Kit, 5,000 assay points

AlphaLISA no-wash immunoassay kit for detection and quantitation of Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture supernatants, and other samples.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
100 assay points
725.00 USD
500 assay points
1585.00 USD
5,000 assay points
10500.00 USD
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Detail Information


  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Brain Derived Neurotrophic Factor (BDNF) is a neurotrophin that acts within the central and peripheral nervous systems. BDNF is expressed in a pro-form as pro-BDNF then later cleaved by plasmin, furin, or pro-convertase enzymes to become the mature form of BDNF. Released BDNF has been observed to bind to both TrkB and LNGFR (p75) receptors. Acting on these receptors, BDNF helps to support the differentiation of new neurons and synapses, promote neuron growth, and generally promote the survival of existing neurons. BDNF has been observed to potentially play a role in several major diseases such as Alzheimer’s, Parkinson’s and Huntington ’s disease. This kit has been designed for the detection and quantification of BDNF in serum, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.


Assay Target BDNF
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Central Nervous System
Unit Size 5,000 assay points