PerkinElmer
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B7-H5/VISTA (human) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® immunoassay kit for human V-domain Ig suppressor of T cell activation (VISTA) enables the quantitative determination of human VISTA (B7-H5, PD-1H) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL3059HV 100 Assay Points 728.00 USD
AL3059C 500 Assay Points 1747.00 USD
AL3059F 5,000 Assay Points 14000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

V-domain Ig suppressor of T cell activation (VISTA), also known as B7-H5 and PD-1H, is a transmembrane protein that functions as an immune checkpoint. VISTA is primarily express in white blood cells and can act either as a ligand or receptor on T-cells. Its main function is to inhibit T cell effector function and maintain peripheral tolerance. VISTA is upregulated in tumor-infiltrating lymphocytes and blocking it with an antibody has proven to stunt tumor growth in mouse melanoma models. HIV-infected individuals also display increased VISTA levels in monocytes.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target B7-H5,VISTA
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 5,000 Assay Points