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AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.

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Product Number Unit Size List Price Your Price Quantity
AL139C 250 µg 1339.00 USD
AL139M 5 mg 7000.00 USD
AL139R 25 mg 24400.00 USD

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Overview

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is methylated at arginine 2(H3R2me) in a homogeneous AlphaLISA assay.

In the AlphaLISA assay, a biotinylated Histone H3 peptide substrate is used in a biochemical enzymatic reaction with histone methyltransferase (HMT) and S-adenosylmethionine (SAM) cofactor. When the enzymatic reaction is stopped, the level of methylation is determined using antibody-coated AlphaLISA Acceptor beads and streptavidin-coated Alpha Donor beads. The streptavidin Donor beads capture the biotin moeity on the peptide substrate, while the antibody on the AlphaLISA Acceptor beads recognizes the specific epigenetic mark (methylation of Histone H3 on arginine 2). Alternatively, a biotinylated Histone H3 (C-term) antibody (Cat. No. AL118) can be used to capture full-length Histone H3 substrates to the streptavidin Donor beads. Upon laser irradiation of the bead complexes at 680 nm, short-lived singlet oxygen molecules produced by the Donor beads can reach the Acceptor beads in proximity to generate an amplified chemiluminescent signal at 615 nm. The intensity of light emission is proportional to the level of substrate modification.

Features:

  • No-wash epigenetic assay
  • Fully-validated mark specificity
  • Substrate flexibility (peptide, protein, histone, nucleosome substrates)
  • Easy-to-automate
  • Fast assay optimization

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugates Anti-H3R2me
Automation Compatible Yes
Bead Type or Core Bead Type AlphaLISA Acceptor
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Methylation
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 5 mg

Resources, Events & More

Resource Type File Name File Format
Brochure EPIGENETICS DRUG DISCOVERY SOLUTIONS PDF 666 KB
Technical Note AlphaLISA PRMT6 Histone H3-Arginine N-methyltransferase Assay PDF 853 KB