The AlphaLISA® immunoassay kit for human Aβ40 enables the detection and quantitation of human Aβ40 in human cerebrospinal fluid and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with other forms of Amyloid beta (i.e. Aβ1-38 and Aβ1-42).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Amyloid beta (Aβ) is a short peptide derived from a transmembrane protein, the amyloid precursor protein (APP), by proteolysis. The β- and γ-secretases cleave the respective N- and C-terminal ends of the Aβ sequence, liberating the Aβ peptide from APP. Aβ40 is the major species of Aβ produced by neurons and other cells, and accounts for over 70% of total Aβ produced, while the remainder is typically composed Aβ42 and other size variants ranging from 36 to 43 AA in length. Interestingly, it has been observed by some that the Aβ40/Aβ42 ratio increased in cerebrospinal fluid of AD patients. Aβ40 could be an important biomarker for detecting Alzheimer’s disease progression. This AlphaLISA kit has been designed to detect Aβ40 in cell culture supernatants and cerebrospinal fluid.
|Assay Target Class||Peptide|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Central Nervous System|
|Unit Size||500 assay points|