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α-tubulin AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for α-tubulin enables the detection and quantitation of human α-tubulin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL383HV 100 assay points 676.00 USD
AL383C 500 assay points 2536.00 USD
AL383F 5,000 assay points 19000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Alpha-tubulin is a 451 amino acid protein that is one of the components of the microtubule cytoskeleton of eukaryotic cells. Alpha and beta tubulin form the skeleton itself, while gamma tubulin is involved in its organization. Alpha-tubulin is ubiquitous in every cell and tissue of the human body, and is highly preserved at the eukaryotic level (human 95% with mouse and 74% with yeast). As such, detection of alpha tubulin in cell extracts is a good tool for normalization of the amount of cell proteins per well in a biochemical assay. The alpha-tubulin AlphaLISA detection kit allows for the detection of alpha-tubulin in tissues homogenates and cell lysates.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target α-tubulin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Dry Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet AlphaLISA a-tubulin detection kit AL383 PDF  563 KB
Event Society of Laboratory Automation & Screening (SLAS) Event