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Alpha-fetoprotein (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Alpha-Fetoprotein (AFP) Detection Kit is designed for detection and quantitationof human AFP in cell culture medium and serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number List Price Your Price Quantity
AL230C 1433.00 USD

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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Alpha-fetoprotein (AFP) is a 67 kDa plasma glycoprotein, present in monomeric, dimeric and trimeric forms, produced by the yolk sac and the fetal liver during fetal life. Thought to be the fetal equivalent of serum albumin, it binds to estradiol, copper, nickel, fatty acids, and bilirubin. It serves as a carrier protein in the blood. AFP expression in adults is often associated with hepatoma or teratoma, as normal adult levels are low, but detectable. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target AFP
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Cancer
Unit Size 500 assay points