The AlphaLISA® Human Alpha-Fetoprotein (AFP) Detection Kit is designed for detection and quantitationof human AFP in cell culture medium and serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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Alpha-fetoprotein (AFP) is a 67 kDa plasma glycoprotein, present in monomeric, dimeric and trimeric forms, produced by the yolk sac and the fetal liver during fetal life. Thought to be the fetal equivalent of serum albumin, it binds to estradiol, copper, nickel, fatty acids, and bilirubin. It serves as a carrier protein in the blood. AFP expression in adults is often associated with hepatoma or teratoma, as normal adult levels are low, but detectable. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Data Sheet||Manual AlphaLISA Human AFP AL230||PDF 210 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|