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α2-Macroglobulin (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human α2-Macroglobulin Detection Kit is designed for detection and quantitation of human α2-Macroglobulin in urine, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number Unit Size List Price Your Price Quantity
AL277C 500 assay points 2745.00 USD
AL277F 5,000 assay points 21500.00 USD

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  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Alpha2-Macroglobulin (a2-Macroglobulin) is a glycoprotein that circulates at a constant level in the blood as a tetramer composed of 4 identical subunits of about 180 kDa. a2-Macroglobulin is mainly secreted by the liver / hepatocytes, but also macrophages, fibroblasts, astrocytes, sertoli cells, adrenocortical cells and certain types of tumors. a2-Macroglobulin is a member of the macroglobulin protein family with similar function: inhibitors of all classes of endopeptidases. a2-Macroglobulin mediates the clearance of endopeptidases from the plasma, but in some cases can also act as a carrier protein in blood for interleukin-6. An abnormal increase of a2-Macroglobulin in blood has been suggested to be associated with multiple sclerosis, glomerular disease, and liver diseases.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.


Assay Target α2 Macroglobulin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Cardiovascular
Unit Size 500 assay points