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AlphaScreen SureFire p-VEGF2 Receptor (Tyr1175) research kit, 10,000 points

The AlphaScreen® SureFire® p-VEGFR2 Receptor (p-Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF2 Receptor (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.

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Product Number List Price Your Price Quantity
TGRVRS10K 6800.00 USD

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Overview

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

Specifications

Assay Pathway Receptor signaling
Assay Target VEGF2 Receptor
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Phosphorylation
Product Brand Name AlphaScreen SureFire
Second Messenger Release Protein phosphorylation
Shipping Condition Blue Ice
Unit Size 1 Kit

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha Technology Solutions PDF 4 MB
Application Note Quick Guide to AlphaScreen SureFire Assay Optimization PDF 307 KB
Manual AlphaScreen® SureFire® phospho-VEGF receptor 2 (Tyr1175) Kit PDF 128 KB
Manual Protocol for AlphaScreen® SureFire® VEGFR2 (p-Tyr1175) Assay Kits PDF 322 KB
Flyer Alpha SureFire Technology PDF 720 KB