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AlphaScreen SureFire Total Topo Iia Assay Kit - 50,000 Points

The AlphaScreen® SureFire® total Topoisomerase II assay is a sandwich immunoassay for quantitative detection of Topoisomerase II (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

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Part Number Unit Size List Price Your Price Quantity
TGRTT2AS500 500 assay points 1449.00 USD
TGRTT2AS10K 10,000 assay points 6400.00 USD
TGRTT2AS50K 50,000 assay points 15700.00 USD
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Overview

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

Specifications

Assay Target Topo IIα
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaScreen SureFire
Shipping Condition Blue Ice
Target Species Human
Unit Size 50,000 assay points

Resources, Events & More

All (7)
Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Brochure Handle Large Biomolecular Interactions With Ease PDF  798 KB
Application Note Quick Guide to AlphaScreen SureFire Assay Optimization PDF  307 KB
Manual AlphaScreen® SureFire® Total Topoisomerase IIa Assay Kits PDF  340 KB
Technical Note Cell lines validated for use with AlphaScreen® SureFire® kits PDF  55 KB
Flyer Alpha SureFire Technology PDF  720 KB
Event Society of Laboratory Automation & Screening (SLAS) Event