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AlphaScreen SureFire Total NFkB p65 Assay Kit - 500 Points

The AlphaScreen® SureFire® total NFκB assay is a sandwich immunoassay for quantitative detection of NFκB (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

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Product Number Unit Size List Price Your Price Quantity
TGRTNFSHV100 1 Kit 385.00 USD
TGRTNFS500 500 assay points 1407.00 USD
TGRTNFS10K 10,000 assay points 6200.00 USD
TGRTNFS50K 50,000 assay points 15200.00 USD

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Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

Assay Pathway NFκB
Assay Target NFκB
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 500 assay points
Product Brand Name AlphaScreen SureFire
Shipping Condition Blue Ice
Unit Size 500 assay points

Resource Library & Events

Resource Type File Name File Format
Application Note Quick Guide to AlphaScreen SureFire Assay Optimization PDF 307 KB
Manual Protocol for AlphaScreen® SureFire® Total NFkB Assay Kits PDF 391 KB
Technical Note Cell lines validated for use with AlphaScreen® SureFire® kits PDF 55 KB
Flyer Alpha SureFire Technology PDF 720 KB