PerkinElmer
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AlphaScreen SureFire Total IkB alpha Assay Kit - 50,000 Points

The AlphaScreen® SureFire® total IκBα assay is a sandwich immunoassay for quantitative detection of IκBα (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

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Part Number
Unit Size
List Price
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TGRTIKS500
500 assay points
1493.00 USD
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TGRTIKS10K
10,000 assay points
6600.00 USD
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TGRTIKS50K
50,000 assay points
16100.00 USD
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Detail Information

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

Specifications

Assay Pathway NFκB
Assay Target IκBα
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaScreen SureFire
Shipping Condition Blue Ice
Target Species Human
Unit Size 50,000 assay points