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AlphaScreen SureFire GAPDH Assay Kit - 10,000 Points

The AlphaScreen® SureFire® total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a cellular normalization assay.

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Product Number List Price Your Price Quantity
TGRGDS10K 6800.00 USD

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Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

Assay Target GAPDH
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Phosphorylation
One Unit Contains 10000 assay points
Product Brand Name AlphaScreen SureFire
Second Messenger Release Protein phosphorylation
Shipping Condition Blue Ice
Unit Size 1 Kit

Resource Library & Events

Resource Type File Name File Format
Manual Protocol for AlphaScreen® SureFire® GAPDH Assay Kits PDF 348 KB
Flyer Alpha SureFire Technology PDF 720 KB