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AlphaScreen SureFire Total Akt1 research kit, 500 points

The AlphaScreen® SureFire® Akt 1 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.

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Product Number Unit Size List Price Your Price Quantity
TGRTA1S500 500 assay points 1543.00 USD
TGRTA1S10K 1 Kit 6800.00 USD
TGRTA1S50K 50,000 assay points 16700.00 USD

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Overview

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

Specifications

Assay Pathway PI3K/Akt/mTOR
Assay Target Akt1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaScreen SureFire
Shipping Condition Blue Ice
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha Technology Solutions PDF 4 MB
Application Note Quick Guide to AlphaScreen SureFire Assay Optimization PDF 307 KB
Manual AlphaScreen® SureFire® total-Akt 1 Kit PDF 142 KB
Manual Protocol for AlphaScreen® SureFire® Akt1 Totak Assay Kits PDF 422 KB
Flyer Alpha SureFire Technology PDF 720 KB