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AlphaScreen SureFire HV Total Akt 1/2/3 Assay Kit - 96-well format

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.

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Part Number Unit Size List Price Your Price Quantity
TGRTAPSHV100 100 assay points 409.00 USD
TGRTAPS500 500 assay points 1493.00 USD
TGRTAPS10K 10,000 assay points 6600.00 USD
TGRTAPS50K 50,000 assay points 16100.00 USD
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Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.


Assay Pathway PI3K/Akt/mTOR
Assay Target Akt1/2/3
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaScreen SureFire
Quantity in a Package Amount 100.0 Units
Shipping Condition Blue Ice
Target Species Human
Unit Size 100 assay points

Resources, Events & More

All (7)
Resource Type File Name File Format
Brochure Alpha product listing PDF  128 KB
Application Note A Practical Approach to Cell Signaling Pathway Analysis PDF  1 MB
Application Note Quick Guide to AlphaScreen SureFire Assay Optimization PDF  307 KB
Manual AlphaScreen® SureFire HV™Akt 1/2/3 Total Assay Kit PDF  335 KB
Poster Expanding the Toolbox of Gamma-irradiated Frozen Cells Validated for GPCR-mediated Kinase Signaling PDF  780 KB
Flyer Alpha SureFire Technology PDF  720 KB
Event Society of Laboratory Automation & Screening (SLAS)