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AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology.

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Product Number List Price Your Price Quantity
TGRMK3S500 1407.00 USD

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Overview

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

Specifications

Assay Pathway MAPK
Assay Target MKK3/6
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Phosphorylation
Product Brand Name AlphaScreen SureFire
Second Messenger Release Protein phosphorylation
Shipping Condition Blue Ice
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Handle Large Biomolecular Interactions With Ease PDF 798 KB
Manual Protocol for AlphaScreen® SureFire® Mkk3 (p-Ser189) /Mkk6 (p-Ser207) Assay Kits PDF 375 KB
Flyer Alpha SureFire Technology PDF 720 KB