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AlphaScreen SureFire HV Akt 1/2/3 (p-Ser473) Assay Kit - 96-well format

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

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Part Number Unit Size List Price Your Price Quantity
TGRA4SHV100 100 assay points 435.00 USD
TGRA4S500 500 assay points 1590.00 USD
TGRA4S10K 10,000 assay points 7000.00 USD
TGRA4S50K 50,000 assay points 17200.00 USD

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Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.


Assay Pathway PI3K/Akt/mTOR
Assay Target Akt1/2/3
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Phosphorylation
Product Brand Name AlphaScreen SureFire
Quantity in a Package Amount 100.0 Units
Second Messenger Release Protein phosphorylation
Shipping Condition Blue Ice
Unit Size 100 assay points