PerkinElmer
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AlphaLISA SureFire Ultra VEGFR2 pY1175 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr1175) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-VEGFR2 assay kit, but can also be ordered separately.

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For research use only. Not for use in diagnostic procedures.

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ALSU-PVGFR-A-L
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Detail Information

This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-VEGF Receptor 2 (Tyr1175) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. This control lysate was prepared from HUVEC cells grown to confluence in T175 flasks, and then serum-starved overnight. The following day the media was removed, and the cells were treated with 100 ng/mL VEGF for 10 minutes. The agonist was removed, and the cells were lysed with 4 mL of freshly prepared 1X Lysis buffer, with shaking for 10 minutes.

Specifications

Assay Target VEGFR2
Assay Target Class Phosphoprotein
Automation Compatible Yes
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice