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AlphaLISA SureFire Ultra Total p38 MAPK Assay Kit - 100 Assay Points

AlphaLISA® SureFire® Ultra Total p38 MAPK assays are sandwich immunoassays for quantitative detection of p38 MAPK (phosphorylated and unphosphorylated) in cellular lysates using Alpha no-wash technology. This assay can be used for normalization of phosphorylation and other studies.

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Product Number Unit Size List Price Your Price Quantity
ALSU-TP38-A-HV 100 assay points 461.00 USD
ALSU-TP38-A500 500 assay points 1558.00 USD
ALSU-TP38-A10K 10,000 assay points 8600.00 USD
ALSU-TP38-A50K 50,000 assay points 26700.00 USD

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The AlphaLISA® SureFire® Ultra™ Total p38 assay is a sandwich immunoassay for quantitative detection of p38 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of protein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates

  • Antibody modulators

  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays

  • Receptor activation studies

  • Screening

Assay Pathway p38 MAPK
Assay Target p38 MAPK
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 1 kit
Product Brand Name AlphaLISA SureFire Ultra
Quantity in a Package Amount 1.0 per pack
Shipping Condition Blue Ice
Unit Size 100 assay points

Resource Library & Events

Resource Type File Name File Format
Manual Protocol for AlphaLISA SureFire Ultra Total p38 MAPK HV (100 point) kit PDF 558 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF 805 KB
Flyer Alpha SureFire Technology PDF 720 KB