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AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Assay Kit - 10,000 assay points

The AlphaLISA® SureFire® Ultra p-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (phosphorylated on Tyr525/526) in cellular lysates using Alpha Technology.

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Part Number Unit Size List Price Your Price Quantity
ALSU-PSYK-A-HV 100 Assay Points 489.00 USD
ALSU-PSYK-A500 500 Assay Points 1653.00 USD
ALSU-PSYK-A10K 10,000 Assay Points 9100.00 USD
ALSU-PSYK-A50K 50,000 Assay Points 28400.00 USD
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  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Disclaimer:  For research use only. Not for use in diagnostic procedures.


Assay Target SYK
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  128 KB
Application Note Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra PDF  2 MB
Manual Protocol for AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Kits PDF  745 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF  805 KB
Flyer Alpha SureFire Technology PDF  720 KB