The AlphaLISA® SureFire® Ultra™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.
The AlphaLISA®SureFire®Ultra™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 (phosphorylated at Tyr693) in cellular lysates using Alpha Technology.
In the AlphaLISA®SureFire®Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA®SureFire®Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Pathway |
JAK/STAT
|
Assay Target Class |
Phospho-protein
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Format |
Microplates
|
Quantity in a Package Amount |
1.0 Units
|
Species |
Human
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
10,000 Assay Points
|