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AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.

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Product Number List Price Your Price Quantity
ALSU-PSM3-A-HV 461.00 USD

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Overview

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.

This kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Pathway TGFß/SMAD
Assay Target SMAD3
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Unit Size 1 kit

Resources, Events & More

Resource Type File Name File Format
Manual Protocol for AlphaLISA SureFire Ultra SMAD3 (p-Ser423/425) HV Assay Kit PDF 548 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF 805 KB
Flyer Alpha SureFire Technology PDF 720 KB