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AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.

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Part Number Unit Size List Price Your Price Quantity
ALSU-PST5-B-HV 100 Assay Points 475.00 USD
ALSU-PST5-B500 500 Assay Points 1605.00 USD
ALSU-PST5-B10K 10,000 Assay Points 8900.00 USD
ALSU-PST5-B50K 50,000 Assay Points 27600.00 USD
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Overview

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology.

In the AlphaLISA® SureFire® Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Pathway JAK/STAT
Assay Target STAT5
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Second Messenger Release Protein phosphorylation
Shipping Condition Blue Ice
Target Species Human
Unit Size 10,000 Assay Points

Resources, Events & More

All (6)
Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra PDF  2 MB
Manual Protocol for AlphaLISA SureFire Ultra pSTAT5 (Tyr694/699) Assay Kits PDF  783 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF  805 KB
Flyer Alpha SureFire Technology PDF  720 KB
Event Society of Laboratory Automation & Screening (SLAS) Event