The AlphaLISA® SureFire® Ultra™ Total mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|ALSU-TMTOR-A-HV||100 Assay Points||475.00 USD|
|ALSU-TMTOR-A500||500 Assay Points||1605.00 USD|
|ALSU-TMTOR-A10K||10,000 Assay Points||8800.00 USD|
|ALSU-TMTOR-A50K||50,000 Assay Points||27600.00 USD|
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In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||50,000 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Application Note||Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra||PDF 2 MB|
|Manual||Protocol for AlphaLISA SureFire Ultra Total mTOR Kit||PDF 740 KB|
|Poster||Rapid homogeneous detection systems for cellular signaling pathways||PDF 805 KB|
|Flyer||Alpha SureFire Technology||PDF 720 KB|