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AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

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Part Number List Price Your Price Quantity
ALSU-PJNK-A500 1605.00 USD
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Overview

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Pathway MAPK
Assay Target JNK1/2/3
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Second Messenger Release Protein phosphorylation
Shipping Condition Blue Ice
Target Species Human
Unit Size 500 Assay Points

Resources, Events & More

All (6)
Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra PDF  2 MB
Manual Protocol for AlphaLISA SureFire Ultra p-JNK 1/2/3 Assay Kits PDF  775 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF  805 KB
Flyer Alpha SureFire Technology PDF  720 KB
Event Society of Laboratory Automation & Screening (SLAS)