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AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

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Product Number List Price Your Price Quantity
ALSU-TERK-A10K 8600.00 USD

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Details

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

This kit contains reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

 

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Assay Pathway MAPK
Assay Target ERK1/2
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
One Unit Contains 1 each
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Unit Size 1 kit

Resource Library

Resource Type File Name File Format
Manual Protocol for AlphaLISA SureFire Ultra Total ERK Assay Kits PDF 784 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF 805 KB
Flyer Alpha SureFire Technology PDF 720 KB