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AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.

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ALSU-PERK-A-HV 461.00 USD

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Details

The AlphaLISA® SureFire® Ultra™ p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.

This kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Assay Pathway MAPK
Assay Target ERK1/2
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
One Unit Contains 1 each
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Unit Size 1 kit

Resource Library

Resource Type File Name File Format
Manual Protocol for AlphaLISA SureFire Ultra ERK1/2 (p-Thr202/Tyr204) Assay Kits PDF 553 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF 805 KB
Flyer Alpha SureFire Technology PDF 720 KB