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AlphaLISA SureFire Ultra Total Akt1 Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

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Product Number Unit Size List Price Your Price Quantity
ALSU-TAKT1-A-HV 100 assay points 461.00 USD
ALSU-TAKT1-A500 500 assay points 1558.00 USD
ALSU-TAKT1-A10K 10,000 assay points 8600.00 USD
ALSU-TAKT1-A50K 50,000 assay points 26700.00 USD

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Overview

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

This kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Pathway PI3K/Akt/mTOR
Assay Target Akt1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Unit Size 100 assay points

Resources, Events & More

Resource Type File Name File Format
Manual Protocol for AlphaLISA SureFire Ultra Total Akt1 HV Assay Kit PDF 558 KB
Poster Rapid homogeneous detection systems for cellular signaling pathways PDF 805 KB
Flyer Alpha SureFire Technology PDF 720 KB