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AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.

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Part Number
Unit Size
List Price
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ALSU-PAKT-A-HV
100 Assay Points
489.00 USD
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ALSU-PAKT-A500
500 Assay Points
1653.00 USD
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ALSU-PAKT-A10K
10,000 Assay Points
9100.00 USD
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ALSU-PAKT-A50K
50,000 Assay Points
28400.00 USD
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Detail Information

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Pathway PI3K/Akt/mTOR
Assay Target Akt1/2/3
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra
Quantity in a Package Amount 10000.0 Units
Shipping Condition Blue Ice
Target Species Human
Unit Size 10,000 Assay Points