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AlphaScreen PhosphoSensor Kit, 10,000 assay points

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.

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For research use only. Not for use in diagnostic procedures.

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6760307D
1,000 assay points
384.00 USD
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6760307M
10,000 assay pointsw
2878.00 USD
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Detail Information

Features/Benefits

  • Especially useful for ser/thr kinases for which no antibody exists
  • Measurement of Large Protein Substrates
  • Not restricted to small peptide substrates like TR-FRET or fluorescence polarization assays allowing for more physiologically relevant data and flexibility in assay development
  • Excellent Z' Values
  • Allows successful transfer to high-throughput screening applications
  • All-in-One Well - With No Radioactivity

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym ""Alpha"" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Specifications

Antibody Conjugates Streptavidin/Phosphosensor
Automation Compatible Yes
Molecular Modification Phosphorylation
Product Brand Name AlphaScreen
Quantity in a Package Amount 10000.0 Units
Shipping Condition Blue Ice
Unit Size 10,000 assay pointsw
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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB

Manual

PhosphoSensor User manual M-6760307-03

The following section describes the possible problems which could be encountered when developing an antibody-free kinase assay using the AlphaScreen Technology. If more information is required, please consult your local PerkinElmer technical support division.

PDF 561 KB