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Anti-bovine IgG AlphaLISA Acceptor Beads, 25 mg

AlphaLISA Acceptor beads conjugated to anti-bovine IgG (total) antibody.

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Part Number Unit Size List Price Your Price Quantity
AL165C 250 µg 804.00 USD
AL165M 5 mg 6100.00 USD
AL165R 25 mg 27200.00 USD
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Overview

AlphaLISA Acceptor beads conjugated to anti-bovine IgG (total) antibody. These beads can be used to capture bovine IgG antibodies. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash immunoassays for:



  • Antibody-antigen binding studies

  • Analyte detection assays

  • Biomarker detection assays

  • Antibody detection assays

  • Other immunoassays


In a typical AlphaLISA assay, 1 mg of AlphaLISA Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL final reaction volume. Bead concentration can be adjusted for optimal performance.

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugates Anti-bovine IgG
Automation Compatible Yes
Bead Type or Core Bead Type AlphaLISA Acceptor
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Unit Size 25 mg

Resources, Events & More

All (7)
Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Guide Alpha Protein-Protein Interaction Quick Start Guide PDF  380 KB
Guide AlphaLISA immunoassay development quick guide PDF  1 MB
Guide ELISA to AlphaLISA Immunoassay Conversion Guide PDF  1 MB
Guide User's Guide To Alpha Assays Protein:Protein Interactions PDF  3 MB
White Paper Alpha Technologies for Antibody Detection and Characterization PDF  534 KB
Event Society of Laboratory Automation & Screening (SLAS) Event